This dataset contains three directories, UPLC-MS contains the raw data and results of ultra-performance liquid chromatography/tandem mass spectrometry, fa is the raw data generated by Bisulfite-PCR and methy_info is the methylation level for Bisulfite-PCR and BS in the randomly selected genomic regions. UPLC-MS directory: mzML raw chromatogram files, excel file for raw data and pdf file for results fa directory: fa file of each samples format: >ID sequence methy_info directory: methylation rate of C on the PCR regions. Both BSP and BS values are shown in the files. format: Scaffold name in the file designation:such as Me_BSP_BS.ABIR02000918.1.region1.txt Scaffold name:ABIR02000918.1 Each column: 1:position: C postion on the Scaffold 2:methylation rate of adults(AD) sample detected by BSP. 3:methylation rate of adults(AD) sample detected by BS-seq. 4:methylation rate of muscle larvae(ML) sample detected by BSP. 5:methylation rate of muscle larvae (ML) sample detected by BS-seq. 6:methylation rate of new-born larvae (NBL) sample detected by BSP. 7:methylation rate of new-born larvae (NBL) sample detected by BS-seq. 8:methylation type (CG/CHG/CHH).